Separation of Detoxin Complex and Characterization

Preliminary work by paper and thinlayer chromatography have indicated the existence of a number of similar antagonists with different specific activity. In the earlier stage of the purification, it was studied whether the minor components were real or artifacts. We found it convenient to use buffered resin chromatography according to the procedure of Moore et al.2) for the separation of detoxin complex into eight groups according to the basicity of the components. Detoxin complex (5.0g, 530u/mg) was chromatographed on a column of Dowex 50Wx2 (4x150cm) at a flow rate of 150 ml/hour. Before use, the resin was equilibrated with 0.2 M pyridine-acetic acid buffer